Rui Huang, Bo Xu
Codsiga R & D Center
Gogoldhig
Pettadu waa xarun ay ka kooban tahay amino acids, oo mid walbaa leh guryo jir ahaan iyo kiimiko oo gaar ah awgood noocyada kala duwan iyo kala dambeynta haraaga amino acid ee ka kooban isku xigxiga. Iyada oo la horumarinayo synthes-ka kiimikada adag ee isku-darka kiimikada adag, isku-darka kiimikada ee ka mid ah pept ee kaladuwan ayaa horumar weyn ka dhigay. Si kastaba ha noqotee, sababta oo ah halabuurka adag ee pettadu waxay ka heleen pettide-ka ee ay ka heleen squesis adag, sheyga ugu dambeeya waa in la nadiifiyaa qaabab kala go 'oo la isku halleyn karo. Hababka nadiifinta ee caadiga ah ee loo adeegsado ee loo yaqaan 'Pept' ee loo yaqaan 'Pept' waxaa ka mid ah ION Isuduwaha Chromatraphy (IEC) oo dib udhacey qalabka korantada ee muujinta ee muuqaalka ah, oo ah kii kiiska sare ee ka-goynta muuqaalka yar yar (MW <1 kda), waxaa horay u soo daabacay Stantai. Teknolojiyada, kaas oo ay tahay sepeflash rp c18 Karttridge c18 Karttridge-ka C18 loo adeegsaday daahirinta deg-degga ah ee tapmopestin (TP-5) iyo kulanka wax soo saarka ee bartilmaameedka ah shuruudaha la helay.
Jaantus 1. 20 Acids amino acids amino oo caadi ah (oo laga soo saaray www.bachm.com).
Waxaa jira 20 nooc oo acids amino acids ah oo caan ku ah qaabka loo yaqaan 'Pept'. Acids-kaan amino waxaa loo qaybin karaa kooxaha soo socda iyadoo loo eegayo hantidooda iyo hantida aashitada ee aashitada: non-poar (hydrophobic), aashitar (sida lagu muujiyey), sida lagu muujiyey Jaantuska 1). Isku xigxiga peptide, haddii amino acids-ka uu yahay mid ka mid ah isku xigxiga ayaa inta badan ah kuwa wax lagu dhejiyo (sida lagu calaamadiyey midabka casaanka ah ee Jaantuska 1). Intii lagu gudajiray hanaanka hubinta ee muunadaha loo yaqaan 'Polar' ee loo yaqaan 'pept' ee loo yaqaan 'polar peptides' ee loo yaqaan 'chromase', ifafaale loo yaqaan 'hyase chrography ph ph ph ph ph ph ph techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn techn ph techn ph col ph ph phedededededed.. And............. Coll.......................... Marka la barbar dhigo sadarka caadiga ah ee C18, safka hore ee C18AQ wuxuu ku habboon yahay daahirinta sanbabka xoogga leh ama biyaha ku jira. Qoraalkan, polar xoog leh oo polar ah ayaa loo adeegsaday tusaalaha oo lagu nadiifiyey sadarka C18AQ. Natiijo ahaan, la kulanka wax soo saarka bartilmaameedka ayaa la kulmay shuruudaha la helay waxaana loo isticmaali karaa cilmi-baarista iyo horumarka soo socda.
| Qalab | Sebalbeen™Mashiinka 2 | |||
| Cartroid | 12 g sepaflash C18 rp oo Flash Shuttridge (spherica Silica, 20 - 45 ° μm, 100 Å 5222222-012-sp) | 12 g sepaflash c18aq rp flafffridge (spersical), 20 - 45 μm, 100 Å 52222-012-sp (AQ)) | ||
| Shaab maamuus | 254 NM, 220 NM | 214 NM | ||
| Wajiga mobilada | KOOXDA A: Biyo Solvent B: acetonitrile | |||
| Sicirka qulqulka | 15 ml / min | 20 ml / min | ||
| Muunad qaadida | 30 mg | |||
| Heer deg-degoobid | Waqtiga (CV) | XULASHADA B (%) | Waqtiga (min) | XULASHADA B (%) |
| 0 | 0 | 0 | 4 | |
| 1.0 | 0 | 1.0 | 4 | |
| 10.0 | 6 | 7.5 | 18 | |
| 12.5 | 6 | 13.0 | 18 | |
| 16.5 | 10 | 14.0 | 22 | |
| 19.0 | 41 | 15.5 | 22 | |
| 21.0 | 41 | 18.0 | 38 | |
| / | / | 20.0 | 38 | |
| 22.0 | 87 | |||
| 29.0 | 87 | |||
Natiijooyinka iyo wada hadalka
In la isbarbar dhigo waxqabadka daahirinta ee muunadda polar pet petfiid ah ee u dhexeeya safka caadiga ah ee 'C18 safka caadiga ah ee C18 iyo sadarka C18AQ, waxaan u isticmaalnay sadarka C18 ee caadiga ah ee loo yaqaan' Flash 'bilowga. Sida ka muuqata Jaantuska 2, sababtuna tahay isbadalka wejiga ee dareeraha ah ee sababaha saaraha C18 ee sababay saamiga aqueous sare, muunadda waxaa si qunyar ah loogu hayey oo uu si toos ah u ruxday wejiga gacanta. Natiijo ahaan, muunadda si hufan looma kala saarin oo la daahirin.
Jaantus 2. Tooshka Tooska ah ee muunadda ee muunadda ku jirta kartoonada C18 ee caadiga ah.
Marka xigta, waxaan u isticmaalnay sadarka C18AQ ee loogu talagalay xoqidda Flash ee muunadda. Sida ka muuqata Jaantuska 3, peptide-ka waxaa si wax ku ool ah loogu hayaa tiirka ka dibna la soo saaray. Shayga bartilmaameedka ah ayaa laga soocay nijaasuhu ee muunadda cayriin iyo aruurinta. Ka dib markii ay ka dib ka dib wada falanqeeyeen HPLC, badeecada la daahiriyey wuxuu daahirsanaada 98.2% waana laga sii faafin karaa daraasadda soo socota iyo horumarka.
Jaantus 3. Tooshka Tooshka Flash ee muunadda ee saamiga C18AQ Chrotridge.
Gunaanad, sepaflash c18aq rp flash cartridge oo ay weheliso service-ka Flash-ka ee Self-ka Sepabean™Mashiinku wuxuu siin karaa xalka degdega ah oo wax ku ool ah oo loogu talagalay nadiifinta sanbabka xoogga leh ama samafalka biyaha ku jira.
Waxaa jira taxane ah oo ah sepeplash c18aq c18aq rp flafder kartoonnada iyada oo sharraxaad ah oo kala duwan oo ka socda tikniyoolajiyadda Statai (sida lagu muujiyey Shaxda 2).
| Lambarka Shayga | Cabbirka sadarka | Sicirka qulqulka (ml / min) | Max.pressure (Psi / bar) |
| SW-5222-004-sp (AQ) | 5.4 g | 5-15 | 400 / 27.5 |
| SW-5222-012-Sp (AQ) | 20 g | 10-25 | 400 / 27.5 |
| Sw-5222-025-sp (AQ) | 33 g | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 g | 15-30 | 400 / 27.5 |
| SW-5222-080-SP (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-sp (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-sp (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-SP (AQ) | 420 g | 40-80 | 250 / 17.2 |
Jadwalka 2aad Qalabka xirxirayaasha
Macluumaad dheeri ah oo ku saabsan faahfaahinta faahfaahinta ee mashiinka sepabeanean ™, ama macluumaadka dalabka ee taxanaha sepaflash-ka Flash-ka Flash Flash-ka, fadlan booqo websaydhka.
Waqtiga Post: Oct-12-2018