Riti Huang, Bol Xu
Noi r & d waena
Hōʻikeʻike
ʻO kahi pomde kahi hui pūʻia o nā amino i hui pūʻia, kēlā me kēia o nā waiwai pilikino a me keʻano o nāʻano likeʻole a me keʻano o nāʻano likeʻole. Me ka hoʻolālāʻana o ka Synthes Stornahi Storyhes, ua holomua ka synthesis o nā pepti maʻamau o nā paipu mau maka likeʻole. Eia nō naʻe, ma muli o ka hoʻopiʻi paʻakikī o ka peptide i loaʻa e ka spise solid baise, pono e hoʻomaʻemaʻeʻia ka huahana hope. ʻO nā hana maʻamau maʻamau i hoʻohanaʻia no nā peptides e pili ana i ka hoʻololiʻana o ka chrisitography ʻO nāʻenehana hana, ma kahi e hoʻohanaʻia ai kahi stocrill rp c18 carridge no ka hoʻomaʻemaʻe wikiwikiʻana (TP-5) a me ka hālāwai huahana e loaʻa ai nā pono.
Kiʻi 1. 20ʻO 20 mau amino mau anino hui (hana hou mai www.bachem.com).
Aia nā 20 mauʻano o nā maʻi amino e pili ana i keʻano o nā peptides. Hiki ke hoʻokaʻawaleʻia kēia mau amino i nā hui e like me kā lākou polarity a me kā lākou waiwai waiwai. I kahiʻano peptide, ināʻo nā amino e hoʻopili ana i keʻano he mau mea nui loa I ka wā o ke kaʻina hana hoʻomaʻemaʻe no kēia mau popo mau popo i ka christ-phromogic Ua hoʻohālikelikeʻia me nā kolamu maʻamau c18,ʻoi aku ka maikaʻi o nā kolamu C18Aq C18AQ i kūpono no ka hoʻomaʻemaʻeʻana i nāʻano polar a iʻole nā mea hydrophilic o nā pola I kēia pou, ua hoʻohanaʻia kahi peptide ikaika e like me ka lapalapa a hoʻomaʻemaʻeʻia e kahi kolamu C18AQ. Ma muli o kahi hopena, ua loaʻa ka manaʻo huahana i loaʻa i nā koi a hiki ke hoʻohanaʻia i kēia noiʻi a me ka hoʻomohalaʻana.
| Hana | Sepabean™Hui 2 | |||
| Nā cartridges | 12 GRerFlash C18 RP Flash Cartridgeʻo (SMIKIP Silica, 20 - 456 μm, 100 å, Kauoha: SW-5222-012-SP) | 12 GRAIPLLASHS C18AQ RP Flash Cartridge, 20 - 456, 100 Å-5222-012-Aq) | ||
| Hauhā | 254 NM, A 220 NM | 214 nm | ||
| ʻO ka pana kelepona | E hoʻopau i kahi: wai Solvend B: Actonitrile | |||
| Ka Holo Waike | 15 ml / min | 20 ml / min | ||
| Mālama i ka hoʻoukaʻana | 30 mg | |||
| Kahuna | Manawa (cv) | Soldnt B (%) | Manawa (min) | Soldnt B (%) |
| 0 | 0 | 0 | 4 | |
| 1.0 | 0 | 1.0 | 4 | |
| 10.0 | 6 | 7.5 | 18 | |
| 12.5 | 6 | 13.0 | 18 | |
| 16.5 | 10 | 14.0 | 22 | |
| 19.0 | 41 | 15.5 | 22 | |
| 21.0 | 41 | 18.0 | 38 | |
| / | / | 20.0 | 38 | |
| 22.0 | 87 | |||
| 29.0 | 87 | |||
Hopena a me nā kūkākūkā
E hoʻohālikelike i ka hana hoʻomaʻemaʻe no ka Polar Peptide ma waena o ka c18 kolamu a me C18aq collun no ka papaʻaina C18AQ. E like me ka hōʻikeʻana ma ke kiʻi 2, ma muli o ke kahe hydrophobic o nā c18 mau kaulahao i laweʻia e keʻano o ka ch18 a ua laweʻia i keʻano o ka C18 a ua pioʻia e keʻano o ke kelepona. Ma muli o kahi hopena,ʻaʻole i hoʻokaʻawaleʻia ka hoʻohālike a hoʻomaʻemaʻeʻia.
Kiʻi 2.ʻO ka Flash Chromatogram o keʻano o ka hoʻohālike ma kahi c18 creridge maʻamau c18.
Ma hope aʻe, ua hoʻohana mākou i kahi kolamu C18AQ no ka Fland Flash o ka hoʻohālike. E like me ka hōʻikeʻana ma ke kiʻi 3, ua hoʻomau maikaʻiʻia ka peptide i ka kolamu a laila e hemo. Ua hoʻokaʻawaleʻia ka huahana Target mai nā meaʻino i loko o keʻano o ka raw a hōʻiliʻiliʻia. Ma hope o ka lyopophilization a laila loiloiʻia e HPLC, ua hoʻomaʻemaʻeʻia ka huahana o 98.2% a hiki ke hoʻohana houʻia
Kiʻi 3.ʻO ka Flash Chromatogram o ka simple ma kahi pahu c18aq.
I ka hopena, hoʻokaʻawale iā C18AQ RP Flash Carridge hoʻohuiʻia me ka'ōnaehana Flash Chromano™Hiki i ka mīkini ke hāʻawi i kahi hopena wikiwiki a me ka hopena no ka hoʻomaʻemaʻeʻana i nā'āpana o nā polar ikaika a iʻole hydrophilinic.
Aia kekahi mau papa o ka moku i hoʻokaʻawale iā C18AQ RP Flash Carridges me nā kiko'ī likeʻole mai kaʻenehana Satai (e like me ka hōʻikeʻana ma ka Papa 2).
| Helu helu | Ka nui o ka kolamu | Ka Holo Waike (ml / min) | Max.pressure (psi / bar) |
| SW-5222-004-SP (AQ) | 5.4 G | 5-15 | 400 / 27.5 |
| SW-5222-012-SP (AQ) | 20 g | 10-25 | 400 / 27.5 |
| SW-5222-025-SP (AQ) | 33 G | 10-25 | 400 / 27.5 |
| SW-5222-040-SP (AQ) | 48 G | 15-30 | 400 / 27.5 |
| SW-5222-080-sp (AQ) | 105 g | 25-50 | 350 / 24.0 |
| SW-5222-120-SP (AQ) | 155 g | 30-60 | 300 / 20.7 |
| SW-5222-220-SP (AQ) | 300 g | 40-80 | 300 / 20.7 |
| SW-5222-330-aq) | 420 g | 40-80 | 250 / 17.2 |
Papa 2. Hoʻokaʻawale i nā c18aq rp flash cardidges. Nā Mea'Ikuhi
No kaʻike hou aku ma nā kiko'ī kiko'ī o Sectivations o Secubean ™ mīkini, a iʻole ka'ōlelo kauoha ma Securflash Serings, e kipa aku i kā mākou pūnaewele.
Post manawa: Oct-12-2018