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Porogaramu R & D Centre
Intangiriro
Ion Guhana Chromatography (IEC) ni uburyo bwa chromatografi busanzwe bukoreshwa no kweza ibice bitangwa muburyo bwa ionic mubisubizo. Nk'uko ibisabwa bitandukanye, ibirego bitandukanye bya ION, IEC irashobora kugabanywamo ubwoko bubiri, cation ihana chromatografiya na anion ihana chromatografiya. Muri cation Guhana chromatografiya, amatsinda ya acide arahujwe hejuru yitangazamakuru ritandukana. Kurugero, aside sulfonic (-o3h) nitsinda rikoreshwa muburyo bwo guhanahana amakuru (scx), nitsinda ryinshi ryabaswe - kandi itsinda ryishyuwe -sox rero rero rishobora gutanga ubundi bwimirimo. Muri Anion Guhana chromatografiya, amatsinda ya alkaline yahujwe hejuru yibitangazamakuru byo gutandukana. Kurugero, amine idasanzwe (-Nr3oh, aho r ari itsinda rya hydrocarbon) mubisanzwe bikoreshwa muburyo bukomeye bwa anion (sax), hamwe nitsinda ryiza-kandi rishobora gutanga izindi anoni.
Mu bicuruzwa bisanzwe, flavonoide yakwegereye abashakashatsi kubera uruhare rwabo mu gukumira no kuvura indwara z'umutima. Kubera ko molekile ya flavooid ari acide kubera kuba hari amatsinda ya hhenolicaxy, ion guhana chromatografiya ni ubundi buryo bwongeyeho icyiciro gisanzwe cyangwa icyiciro cyanditseho chromatografiya kugirango utandukane no kwezwa ibyo bigo bya acide. Muri flash chromatography, itangazamakuru risanzwe rikoreshwa muguhuza ion ni Silica gel matrix aho ishyano rya gion rihanaguwe hejuru. Imyitozo ikunze gukoreshwa ion ihanamo kuri flazutografi na scorf (mubisanzwe acide sulfonic tsinda acide) na sax (mubisanzwe amine yitsinda). Muburyo bwasohoye mbere yo gusabana numutwe "Gusaba gutandukanya Cation Guhana chromatografi mu kwezwa kw'inkingi za alromato" na tekinoroji ya santatografi "na tekinoroji ya Santai, inkingi za Scor zakoreshejwe mu kwezwa. Muri iyi nyandiko, uruvange rwamahame ataboganwa kandi acide yakoreshejwe nkicyitegererezo cyo gusuzuma ishyirwa mubikorwa ryinkingi za Sax mugusukura ibigo bya acide.
Icyiciro cyageragejwe
Igishushanyo 1. Igishushanyo cya Schematic cyicyiciro gihagaze cyahujwe hejuru yitangazamakuru ritandukanya SAX.
Muri iyi nyandiko, inkingi ya Sax yabanjirije hamwe na amine ya amine yinguzanyo ya silika yakoreshejwe (nkuko bigaragara ku gishushanyo 1). Uruvange rwa chromine na 2,4-diydrixybenzoic aside yakoreshejwe mugihe icyitegererezo cyo kwezwa (nkuko bigaragara ku gishushanyo cya 2). Uruvange rwashonze muri methanol kandi rwikorewe kuri karitsiye ya flash na injiji. Gukora igerageza rya flash ryerekanwe ku mbonerahamwe ya 1.
Igishushanyo cya 2. Imiterere yimiti yibice bibiri muruvange ruvanze.
| Igikoresho | Sepabean ™ imashini t | |||||
| Amakarito | 4 G DissFlash Urukurikirane rusanzwe Flash Cartridge (Ubucucike bwa Silica, 40 - 63 μm, 60 å, gahunda ya gahunda: s-51014) | 4 G DissFlash Borwantuse Yex Flash Cartridge (Ubucucike bwa Silica, 40 - 63 μM, | ||||
| Uburebure | 254 nm (gutahura), 280 nm (gukurikirana) | |||||
| Icyiciro cya mobile | Gukemura a: n-hexane | |||||
| Solven B: Ethyl Acetate | ||||||
| Igipimo | 30 ml / min | 20 ml / min | ||||
| Icyitegererezo | 20 mg (uruvange rwibigize a kandi ibice b) | |||||
| Gradient | Igihe (CV) | Yamazaki B (%) | Igihe (CV) | Yamazaki B (%) | ||
| 0 | 0 | 0 | 0 | |||
| 1.7 | 12 | 14 | 100 | |||
| 3.7 | 12 | / | / | |||
| 16 | 100 | / | / | |||
| 18 | 100 | / | / | |||
Ibisubizo n'ibiganiro
Ubwa mbere, uruvange ruvanze rwatandukanijwe nicyiciro gisanzwe cya flash cartrige mbere yubucuruzi hamwe na silica isanzwe. Nkuko bigaragara ku gishushanyo cya 3, ibice bibiri murugero byasohowe bivuye mu Ikarita imwe. Ibikurikira, cartridge ya Sax Flash yakoreshejwe kugirango isuku. Nkuko bigaragara ku gishushanyo cya 4, ibice bya aside b byagumishijwe rwose kuri SAX Cartridge. Ibice bitabogamye AURVULS yahise yavaga mu makarito hamwe no kwishyuza icyiciro cya mobile.
Igishushanyo cya 3. Flash chromatogram yicyitegererezo kumurongo usanzwe wa cartridge isanzwe.
Igishushanyo cya 4. Flash chromatogram yumutego kuri cartridge ya sax.
Kugereranya Igishushanyo cya 3 nishusho 4, ibice a ifite imiterere idahwitse kuri cartridge ebyiri zitandukanye. Kugirango twemeze niba impinga yo hejuru ihuye nibigize, turashobora gukoresha ibintu byuzuye byuzura bikaba byubatswe muri software igenzura rya Sepabeyan ™ imashini. Fungura amakuru yubushakashatsi bwibinyuranye byombi, Kurura kumurongo wa Netrators mugihe cya chromatogram kugeza kumanota ajyanye ningingo zombi zijyanye ningingo ya chromatogram (nkuko bigaragara ku gishushanyo cya 5 nigishushanyo cya 6). Kugereranya uburebure bwuzuye bwamakuru yububiko bwibintu byombi, ibice a bifite uburyo bujyanye nubushakashatsi buhoraho. Kubwimpamvu yigice a ifite imiterere idahuye kumagare abiri ya flash atandukanye, biratangaje ko hari umwanda wihariye mubice bifite igihe cyo kugumana gitandukanye kuri cartridge isanzwe hamwe na Sax Cartridge. Kubwibyo, urutonde rukora amajwi rutandukanye kubigize a no kwanduye kuri makarito yombi ya flash, bivamo imiterere itemewe kuri chromatograms.
Igishushanyo cya 5. Uburebure bwuzuye bwikigize A kandi umwanda utandukanijwe nicyiciro gisanzwe cya Cartridge.
Igishushanyo cya 6. Uburebure bwuzuye bwikigize igice cya kabiri cyatandukanijwe na SAX Cartridge.
Niba intego yibicuruzwa bikusanyirizwa hamwe nibice bitabogamye a, umurimo wo kweza urashobora kurangira byoroshye ukoresheje extridge ya Sax Ikarita yo kwikuramo icyitegererezo. Kurundi ruhande, niba intego yibicuruzwa bikusanyirizwa hamwe nibice bya aside b, uburyo bwo gufatwa bushobora kwemezwa gusa mubice bisanzwe byimpande zibisanzwe: Iyo icyitegererezo cyakuweho kuri methanol igisubizo kirimo aside 5%. Ions ya Arcetate muri prose igendanwa izarushaho guhangana nibigize B kugirango iyobore amatsinda ya aine ya amine kumurongo uhagaze, bityo asohora ibice b kuva kuri karitsiye kugirango abone ibicuruzwa. Chromatogram yicyitegererezo yatandukanijwe muburyo bwo guhana ion bwerekanwe ku gishushanyo cya 7.
Igishushanyo cya 7. Flash chromatogram yibice B yatinze muburyo bwa ion yo guhana kuri Sax Cartridge.
Mu gusoza, intangarugero cyangwa itaboutet Byongeye kandi, hamwe nubufasha bwumurongo wuzuye urwenya rwubatswe muri software igenzura rya Sepabean ™ imashini irambuye yagereranijwe kandi yemeje ko ibintu bimaze kugereranywa no kwezwa vuba kandi bitunganijwe neza.
| Umubare w'ikintu | Ingano y'inkingi | Igipimo (ML / Min) | Max.kwishimira (PSI / BAR) |
| Sw-5001-004-ir | 5.9 g | 10-20 | 400/1 27.5 |
| Sw-5001-012-ir | 23 g | 15-30 | 400/1 27.5 |
| Sw-5001-025-ir | 38 g | 15-30 | 400/1 27.5 |
| Sw-5001-040-ir | 55 g | 20-40 | 400/1 27.5 |
| Sw-5001-080-ir | 122 G. | 30-60 | 350 / 24.0 |
| Sw-5001-120-ir | 180 g | 40-80 | 300 / 20.7 |
| Sw-5001-220-ir | 340 g | 50-100 | 300 / 20.7 |
| Sw-5001-330-ir | 475 g | 50-100 | 250 / 17.2
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Imbonerahamwe ya 2. Gutandukanya Borse Urukurikirane rwa Sax Flash Cartridges. Ibikoresho byo gupakira: ultra-kurebera SAX-induru ya silica, 40 - 63 μm, 60 å.
Kubindi bisobanuro bijyanye nibisobanuro birambuye bya Sepabean ™Imashini, cyangwa amakuru yo gutumiza kuri DissFlash Urukurikirane rwa Flash Cartridges, nyamuneka sura urubuga rwacu.
Igihe cyohereza: Nov-09-2018