Ibibazo

Sukura umutwe wa filteri yumutwe rwose kugirango ukureho umwanda wose, Nibyiza gukoresha isuku ya ultrasonic.

1. selile selile ya detector yaranduye.

2. Ingufu nke ziva mumucyo.

3. Ingaruka za pompe.

4. Ingaruka yubushyuhe bwa detector.

5. Hano hari ibibyimba muri pisine.

6. Inkingi cyangwa mobile mobile yanduye.

Mugutegura chromatografiya, umubare muto wurusaku rwibanze ntacyo ruhindura kubitandukanya.

1. Umuyoboro uhuza inyuma yimashini urekuye cyangwa wangiritse;Simbuza umuyoboro uhuza;

2. Inzira yo kugenzura gaze yangiritse.Simbuza cheque.

Nyuma yo gutandukana, birakenewe gutegereza iminota 3-5 mbere yo kuzimya kugirango hamenyekane ubusugire bwibisobanuro byubushakashatsi.

Kuringaniza inkingi birashobora kurinda inkingi kwangizwa ningaruka zidasanzwe iyo solvent ihita inyura mu nkingi.Mugihe silika yumye yabanje gupakirwa muminkingi ihura na solvent kunshuro yambere mugihe cyo gutandukana, ubushyuhe bwinshi bushobora kurekurwa cyane cyane mugihe ibishishwa bitemba kumuvuduko mwinshi.Ubu bushyuhe bushobora gutuma inkingi yumubiri ihinduka bityo igashonga kumeneka kuva kumurongo.Rimwe na rimwe, ubu bushyuhe bushobora no kwangiza urugero rwerekana ubushyuhe.

Birashoboka ko byatewe no kubura amavuta yo gusiga ku kizunguruka cya pompe.

Umubare wuzuye wa sisitemu tubing, umuyoboro hamwe no kuvanga chambre ni nka mL 25.

Ingirabuzimafatizo ya module ya detector yandujwe nicyitegererezo gifite imbaraga zikomeye za UV.Cyangwa birashobora guterwa no kwishongora UV nikintu gisanzwe.Nyamuneka kora ibikorwa bikurikira:

1. Kuraho flash inkingi hanyuma usukure sisitemu tubing hamwe na polar solvent ikomeye hanyuma ukurikirwa na polar solvent nkeya.

2 kuri 254 nm iri munsi yubwa n-hexane.Mugihe ibi bintu bibaye, turashobora kubyitwaramo dukanze buto ya "Zero" kurupapuro rwitandukanya rwo gutandukana muri AppaBean App.

3.Ingirabuzimafatizo ya module ya detector yanduye cyane kandi igomba gusukurwa ultrases.

Birashobora guterwa nuko abahuza kumurongo wumutwe kimwe no kumurongo wibanze byabyimbye kugirango uhuze.

Umukoresha arashobora kuzamura intoki abafite inkingi umutwe ukoresheje imbaraga nkeya.Iyo inkingi ifata umutwe yazamuye hejuru yuburebure runaka, inkingi ifata umutwe igomba kuba ishobora kwimurwa no gukora kuri buto kuri yo.Niba inkingi ifata umutwe idashobora kuzamurwa nintoki, uyikoresha agomba kuvugana nubufasha bwa tekinike.

Uburyo bwihutirwa ubundi buryo: Umukoresha arashobora gushiraho inkingi hejuru yinkingi ifata umutwe aho.Icyitegererezo cyamazi gishobora guterwa muburyo butaziguye.Icyitegererezo gikomeye cyo gupakira inkingi kirashobora gushyirwaho hejuru yinkingi yo gutandukana.

1. Ingufu nke zituruka ku mucyo;

2. Ikidendezi kizenguruka cyanduye;Mu buryo bwimbitse, nta mpinga nini cyangwa impinga nini ni ntoya mu gutandukana, Ingufu zerekana imbaraga zifite munsi ya 25%.

Nyamuneka sukuza umuyoboro hamwe na solve ikwiye kuri 10ml / min kuri 30min hanyuma urebe ingufu z'amashanyarazi.Niba nta gihindutse kuri spekiteri, bisa nkimbaraga nke ziva mumucyo, nyamuneka usimbuze itara rya deuterium;Niba ibice byahindutse, pisine izenguruka yanduye ， nyamuneka komeza usukure hamwe na solve ikwiye.

Nyamuneka reba umuyoboro uhuza buri gihe.

Uburebure bwumurambararo bushyirwa kumurongo wa wavle uri munsi ya 245 nm kuva Ethyl acetate ifite kwinjiza cyane kurwego rwo gutahura munsi ya 245nm.Gutembera kwibanze bizaba byiganje cyane mugihe Ethyl acetate ikoreshwa nka eluting solvent hanyuma duhitamo 220 nm nkuburebure bwumurongo.

Nyamuneka hindura uburebure bwumurongo.Birasabwa guhitamo 254nm nkuburebure bwumurongo.Niba 220 nm aribwo burebure bwonyine bukwiranye nicyitegererezo, umukoresha agomba kwegeranya eluent hamwe nubushishozi bwitondewe kandi birashobora gukusanywa muriki kibazo.