Le fa'amamāina o mea e sili ona leaga o le polar i vaila'au fa'ama'i e C18AQ Columns

Mingzu Yang, Bo Xu
Talosaga R&D Nofoaga Autu

Folasaga
O vaila'au fa'ama'i o se vasega o metabolites lona lua e gaosia e ni meaola ninii (e aofia ai siama, sigi, actinomycetes) po'o mea fa'apena fa'apena e fa'apipi'iina i kemikolo po'o semi-synthesized.O vaila'au fa'ama'i e mafai ona taofia ai le tuputupu a'e ma le ola o isi microorganisms.O le antibiotic muamua na maua e le tagata, penicillin, na maua e le British microbiologist Alexander Fleming i le 1928. Na ia matauina e le mafai ona tupu le siama i tafatafa o le limu i totonu o le staphylococcus culture dish lea na afaina i le limu.Na ia fai mai e tatau ona natia e le limu se mea e puipuia ai siama, lea na ia faaigoaina o le penicillin i le 1928. Peitaʻi, o mea aoga e leʻi faʻamamaina i lena taimi.I le 1939, na filifili ai Ernst Chain ma Howard Florey o le Iunivesite o Oxford e fausia se fualaau e mafai ona togafitia ai siama siama.Ina ua uma ona faʻafesoʻotaʻi Fleming e maua mai faʻamaʻi, na latou aveese ma faʻamama le penicillin mai faʻamaʻi.Mo lo latou atinaʻeina manuia o le penicillin e avea o se vailaʻau faʻamalolo, Fleming, Chain ma Florey faʻasoa le 1945 Nobel Prize in Medicine.

O vailaau fa'ama'i e fa'aaogaina e fai ma vaila'au fa'ama'i e togafitia pe puipuia ai fa'ama'i pipisi.E tele vaega autu o vailaʻau faʻaaogaina e avea ma vailaʻau faʻamaʻi: β-lactam antibiotics (e aofia ai penicillin, cephalosporin, ma isi), aminoglycoside antibiotics, macrolide antibiotics, tetracycline antibiotics, chloramphenicol (total synthetic antibiotic), ma isi mea. fermentation biological, semi-synthesis ma le tuufaatasiga atoa.O vailaʻau faʻamaʻi e maua mai i le faʻafefe o meaola e manaʻomia ona faʻaleleia faʻavae e ala ile vailaʻau ona o le mautu o vailaʻau, aʻafiaga oona, alaleo antibacterial ma isi faʻafitauli.A maeʻa ona faʻaleleia faʻamaʻi, e mafai e vailaʻau faʻamaʻi ona ausia le faʻateleina o le mautu, faʻaitiitia aʻafiaga oona, faʻalauteleina alaleo antibacterial, faʻaitiitia le teteʻeina o vailaʻau, faʻaleleia le bioavailability, ma faʻaleleia ai le aʻafiaga o togafitiga fualaau.O le mea lea, o vailaʻau semi-synthetic o loʻo avea nei ma taʻutaʻua sili ona lauiloa i le atinaʻeina o vailaʻau faʻamaʻi.

I le atinaʻeina o vailaʻau faʻamaʻi semi-synthetic, o vailaʻau faʻamaʻi o loʻo i ai meatotino e maualalo le mama, tele o mea faʻatau ma vaega lavelave talu ai e maua mai i mea faʻafefete microbial.I lenei tulaga, o le auʻiliʻiliga ma le puleaina o mea le mama i vailaʻau semi-synthetic e sili ona taua.Ina ia mafai ona faʻamaonia lelei ma faʻamaonia mea leaga, e manaʻomia le maua o le tele o mea leaga mai le gaosiga faʻapipiʻi o vailaʻau semi-synthetic.Faatasi ai ma le masani ona faʻaaogaina le faʻaogaina o le faʻaogaina o metotia, o le flash chromatography o se auala taugofie ma tulaga lelei e pei o le tele o faʻataʻitaʻiga faʻatauga aofaʻi, tau maualalo, taimi faʻasaoina, ma isi.

I lenei pou, o le eleelea autu o le semi-synthetic aminoglycoside antibiotic na faʻaaogaina e fai ma faʻataʻitaʻiga ma faʻamamāina e le SepaFlash C18AQ pusa faʻatasi ma le masini chromatography flash SepaBean ™.O le oloa faʻatatau e fetaui ma manaʻoga na maua ma le manuia, faʻaalia ai se fofo sili ona lelei mo le faʻamamaina o nei mea.