Isuku ryimyanda myinshi cyane muri Antibiyotike na C18AQ Inkingi

Mingzu Yang, Bo Xu
Porogaramu R&D Ikigo

Intangiriro
Antibiyotike ni icyiciro cya metabolite ya kabiri ikorwa na mikorobe (harimo na bagiteri, ibihumyo, actinomycetes) cyangwa ibisa nkibyo bihujwe na chimique cyangwa se kimwe cya kabiri.Antibiyotike irashobora kubuza gukura no kubaho kwizindi mikorobe.Antibiyotike ya mbere yavumbuwe n’umuntu, penisiline, yavumbuwe n’umuhanga mu binyabuzima w’umwongereza witwa Alexander Fleming mu 1928. Yabonye ko bagiteri ziri hafi y’ikibumbano zidashobora gukura mu biryo by’umuco wa staphylococcus byari byanduye.Yavuze ko ifumbire igomba gusohora ibintu birwanya antibacterial, yise penisiline mu 1928. Icyakora, icyo gihe ibintu byakoraga ntibyari byera.Mu 1939, Ernst Chain na Howard Florey bo muri kaminuza ya Oxford bahisemo gukora imiti ishobora kuvura indwara ziterwa na bagiteri.Nyuma yo kuvugana na Fleming kugirango babone imbaraga, bakuyemo neza kandi bahanagura penisiline mumurongo.Kugira ngo bateze imbere penisiline nk'umuti uvura, Fleming, Chain na Florey basangiye igihembo cyitiriwe Nobel mu 1945.

Antibiyotike ikoreshwa nka antibacterial miti yo kuvura cyangwa gukumira indwara ziterwa na bagiteri.Hariho ibyiciro byinshi byingenzi bya antibiotike ikoreshwa nka antibacterial agent: β-antibiyotike ya lactam (harimo penisiline, cephalosporine, nibindi), antibiotike ya aminoglycoside, antibiotike ya macrolide, antibiotike ya tetracycline, chloramphenicol (antibiotique yuzuye ya sintetike), nibindi nibindi. fermentation ya biologiya, igice-synthesis hamwe na synthesis yose.Antibiyotike ikorwa na fermentation ya biologiya igomba guhindurwa muburyo bwa chimique bitewe nubumara bwimiti, ingaruka zuburozi, antibacterial spektr nibindi bibazo.Nyuma yo guhindurwa mu buryo bwa shimi, antibiyotike zishobora kugera ku kongera umutekano, kugabanya ingaruka z’ubumara, kwagura imiti igabanya ubukana, kugabanya imiti, kugabanya bioavailable, bityo bikagira ingaruka nziza zo kuvura ibiyobyabwenge.Kubwibyo, antibiotike ya sintetike ya kimwe cya kabiri nicyerekezo gikunzwe cyane mugutezimbere imiti ya antibiotique.

Mugutezimbere antibiyotike ya kimwe cya kabiri, antibiyotike ifite imiterere yubuziranenge buke, ibicuruzwa byinshi byongeweho nibigize ibintu bigoye kuva bikomoka kuri mikorobe ya fermentation.Muri iki gihe, gusesengura no kugenzura umwanda muri antibiyotike ya kimwe cya kabiri ni ngombwa.Kugirango tumenye neza kandi turanga umwanda, birakenewe kubona umubare uhagije wumwanda uva mubicuruzwa bya sintetike ya antibiotique ya sintetike.Muburyo bukoreshwa muburyo bwo gutegura umwanda, flash chromatografiya nuburyo buhendutse hamwe nibyiza nkamafaranga manini yo gupakira ibintu, igiciro gito, kuzigama igihe, nibindi. Flash chromatografiya yakoreshejwe cyane nabashakashatsi ba sintetike.

Muri iyi nyandiko, umwanda nyamukuru wa kimwe cya kabiri cyogukora aminoglycoside antibiotique yakoreshejwe nkicyitegererezo kandi usukurwa na karitsiye ya SepaFlash C18AQ ihujwe na flash ya chromatografiya ya SepaBean ™ imashini.Intego yibicuruzwa byujuje ibisabwa byabonetse neza, byerekana igisubizo cyiza cyane cyo kweza ibyo bikoresho.