FAQs

Most TLC plates are compatible with common organic solvents (e.g., hexane, ethyl acetate, methanol). Check the product specifications for specific solvent resistance.

• Choose the adsorbent type (e.g., silica or alumina) based on the polarity of your compounds.
• Consider layer thickness (250 µm or 0.25 mm for analytical, 1,000 – 2,000 µm or 1 – 2 mm for preparative).
• Use plates with fluorescent indicators for UV detection.

Standard sizes are 20 x 20 cm, 10 x 10 cm, and 5 x 5 cm. Custom sizes can also be provided upon request.

• Use silica gel for general-purpose and normal-phase separations.
• Use alumina for more basic compounds.
• Select reverse-phase plates for polar compounds or water-based separations.

Pre-coated plates are ready-to-use, offering uniform layers and reproducible results. Self-prepared plates are cost-effective but may vary in layer consistency and require additional preparation time.

TLC plates are used for separating and analyzing mixtures of compounds. Common applications include reaction monitoring, purity testing, and qualitative identification of compounds in pharmaceuticals, food, and environmental samples.

Santai Solvent Lines Kit & Accessories

No, end-capped silica is insoluble in any commonly used organic solvent.

For optimal purification with C18 flash columns, please follow these steps:
① Flush the column with 100% of the strong (organic) solvent for 10 – 20 CVs (column volume), typically methanol or acetonitrile.
② Flush the column with 50% strong + 50% aqueous (if additives are required, include them) for another 3 – 5 CVs.
③ Flush the column with the initial gradient conditions for 3 – 5 CVs.

For column size between 4g and 330g, standard Luer connector is used in these flash columns. For column size of 800g, 1600g and 3000g, additional connector adaptors should be used to mount these large flash columns on the flash chromatography system. Please refer to the document Santai Adaptor Kit for 800g, 1600g, 3kg Flash Columns for more details.

For normal phase column, it is recommended to use the mobile phase where the ratio of methanol does not exceed 25%.

Generally, it is recommended to use the mobile phase where the ratio of the polar solvents does not exceed 5%. The polar solvents include DMSO, DMF, THF, TEA etc.