ʻO ka hoʻomaʻemaʻe ʻana o ka Taxus Extract e SepaBean™ Machine

Meiyuan Qian, Yuefeng Tan, Bo Xu
Ka Papahana R&D Center

Hoʻolauna
ʻO Taxus (Taxus chinensis a i ʻole Chinese yew) kahi mea kanu ʻāhiu i pale ʻia e ka ʻāina.He mea kanu laha ole ia i waihoia mai e ka Quaternary glaciers.ʻO ia wale nō ka lāʻau lapaʻau maoli ma ka honua.Hoʻokaʻawale ʻia ʻo Taxus ma ka ʻāina wela o ka ʻāpana ʻākau a hiki i ka ʻāina waena-subtropical, me kahi o 11 mau ʻano i ka honua.Aia 4 mau ʻano a me 1 ʻano like ʻole ma Kina, ʻo ia ʻo Northeast Taxus, Yunnan Taxus, Taxus, Tibetan Taxus a me Southern Taxus.Hāʻawi ʻia kēia mau ʻano ʻelima ma Southwest China, South China, Central China, East China, Northwest China, Northeast China a me Taiwan.Aia nā mea kanu Taxus i nā ʻano mea kemika, me ka taxanes, flavonoids, lignans, steroids, phenolic acids, sesquiterpenes a me glycosides.ʻO ka lāʻau anti-tumor kaulana ʻo Taxol (a i ʻole Paclitaxel) he ʻano ʻauhau.Loaʻa iā Taxol nā hana anticancer kūikawā.Hiki iā Taxol ke "hoʻokuʻu" i nā microtubules ma o ka hui pū ʻana me lākou a pale i nā microtubules mai ka hoʻokaʻawale ʻana i nā chromosomes i ka manawa o ka hoʻokaʻawale ʻana i nā cell, no laila ke alakaʻi nei i ka make ʻana o nā pūnaewele hoʻokaʻawale, ʻoi aku ka wikiwiki o ka hoʻonui ʻana i nā maʻi kanesa [1].Eia kekahi, ma ka hoʻoulu ʻana i nā macrophages, ʻo Taxol ka mea e hoʻemi ai i ka TNF-α (tumor necrosis factor) receptors a me ka hoʻokuʻu ʻana o TNF-α, ma laila e pepehi ai a i ʻole ke kāohi ʻana i nā cell tumor [2].Eia kekahi, hiki iā Taxol ke hoʻoulu i ka apoptosis ma ka hana ʻana ma ke ala apoptotic receptor i hoʻopili ʻia e Fas / FasL a i ʻole ka hoʻoulu ʻana i ka ʻōnaehana protease cysteine ​​[3].Ma muli o ka nui o ka hopena anticancer, ua hoʻohana nui ʻia ʻo Taxol i ka mālama ʻana i ka maʻi maʻi ovarian, ka maʻi maʻi umauma, ka maʻi maʻi ʻaʻai liʻiliʻi liʻiliʻi (NSCLC), ka maʻi maʻi ʻōpū, ka maʻi maʻi esophageal, ka maʻi maʻi ʻaʻai, ka maʻi prostate, ka melanoma malignant, ke poʻo a me ka ʻāʻī. ka maʻi 'aʻai, etc[4].Maikaʻi no ka maʻi maʻi umauma holomua a me ka maʻi maʻi ovarian holomua, he hopena curative koʻikoʻi ʻo Taxol, no laila ua ʻike ʻia ʻo ia ʻo "ka laina hope o ka pale no ka mālama ʻana i ka maʻi kanesa".

ʻO Taxol ka lāʻau anticancer kaulana loa i ka mākeke honua i nā makahiki i hala iho nei a ua manaʻo ʻia ʻo ia kekahi o nā lāʻau anticancer maikaʻi loa no nā kānaka i nā makahiki he 20 e hiki mai ana.I nā makahiki i hala iho nei, me ka piʻi nui ʻana o ka heluna kanaka a me ka maʻi maʻi maʻi, ua piʻi nui ka koi no Taxol.I kēia manawa, koi ʻia ʻo Taxol no ka noiʻi lapaʻau a i ʻole ka ʻepekema ʻepekema i lawe pololei ʻia mai Taxus.ʻO ka mea pōʻino, haʻahaʻa loa ka ʻike o Taxol i nā mea kanu.No ka laʻana, ʻo ka ʻikepili Taxol he 0.069% wale nō i ka ʻili o Taxus brevifolia, ka mea i manaʻo ʻia he ʻike kiʻekiʻe loa.No ka lawe ʻana i 1 g o Taxol, pono ia ma kahi o 13.6 kg o ka ʻili Taxus.Ma muli o kēia hoʻohālikelike, pono ia he 3 - 12 lāʻau Taxus i ʻoi aku ma mua o 100 mau makahiki e mālama ai i kahi maʻi maʻi maʻi ovarian.ʻO ka hopena, ua ʻoki ʻia ka nui o nā kumulāʻau Taxus, a ua kokoke e hoʻopau ʻia kēia ʻano makamae.Eia kekahi, ʻilihune loa ʻo Taxus i nā kumuwaiwai a lohi i ka ulu ʻana, kahi mea paʻakikī no ka hoʻomohala hou a me ka hoʻohana ʻana iā Taxol.

I kēia manawa, ua hoʻopau maikaʻi ʻia ka huina o Taxol.Eia naʻe, paʻakikī loa kona ala synthetic a me ke kumu kūʻai nui, no laila ʻaʻohe mea koʻikoʻi o ka ʻoihana.ʻO ke ʻano semi-synthetic o Taxol i kēia manawa ke ʻano oʻo a manaʻo ʻia he ala kūpono e hoʻonui ai i ke kumu o Taxol ma waho o ke kanu ʻana.ʻO ka pōkole, i ka semi-synthesis o Taxol, ʻo ka Taxol precursor compound i ʻoi aku ka nui o nā mea kanu ʻo Taxus i unuhi ʻia a laila hoʻololi ʻia i Taxol e ka synthesis kemika.ʻO ka ʻike o 10-deacetylbaccatin Ⅲ i loko o nā nila o Taxus baccata hiki ke piʻi i ka 0.1%.A ua maʻalahi nā nila e hana hou i ka hoʻohālikelike ʻana me nā ʻili.No laila, ʻo ka semi-synthesis o Taxol i hoʻokumu ʻia ma 10-deacetylbaccatin Ⅲ ke ʻimi nei i ka nui o ka nānā ʻana mai nā mea noiʻi [5] (e like me ka hōʻike ʻana ma ka Figure 1).

Kiʻi 1. ʻO ke ala semi-synthetic o Taxol e pili ana i ka 10-deacetylbaccatin Ⅲ.

Ma kēia pou, ua hoʻomaʻemaʻe ʻia ka mea kanu ʻo Taxus e kahi ʻōnaehana chromatography wai hoʻomākaukau uila SepaBean™ i hui pū ʻia me SepaFlash C18 reversed-phase (RP) pahu pahu uila i hana ʻia e Santai Technologies.Ua loaʻa ka huahana pahuhopu e hālāwai me nā koi maʻemaʻe a hiki ke hoʻohana ʻia i ka noiʻi ʻepekema ma hope, e hāʻawi ana i kahi hopena kūpono no ka hoʻomaʻemaʻe wikiwiki ʻana i kēia ʻano huahana kūlohelohe.