SepaPrep™ UNI Series
Universal Reversed-Phase Performance for Routine and Method-Development Workflows
Series Overview
The SepaPrep™ UNI Series is designed as a robust, general-purpose reversed-phase solution for analytical and preparative HPLC applications. Built on high-purity spherical silica and optimized C18 surface bonding, UNI columns deliver stable retention, excellent peak shape, and reliable reproducibility for routine analysis and method development.
The balanced surface chemistry of the SepaPrep™ UNI Series provides predictable selectivity for non-polar to moderately polar compounds, while advanced end-capping minimizes secondary interactions. This design supports consistent performance across repeated injections, between column batches, and during scale-up from analytical to preparative formats.
Key Features
Silica Characteristics
| Phase | USP Code | Particle Size | Pore Size | Specific Surface Area | Endcapping | Carbon Load | pH Range |
|---|---|---|---|---|---|---|---|
| C18 | L1 | 3 & 5 µm | 120 Å | 340 m2/g | Double | 14 % | 1.5 - 9.0 |
To maintain consistent performance and extend the working life of the column, it is recommended to operate the SepaPrep™ UNI C18 within its optimal pH range of 2.0 to 6.0. The maximum operating temperature is 60°C and the maximum pressure rating is 5,800 psi (40 MPa). For routine operation, pressure below 2,900 psi (20 MPa) is recommended.
Ordering Information
| Column Type | ID × Length | Phase | Particle Size | Pore Diameter | Part Number |
|---|---|---|---|---|---|
| Analytical | 4.6 × 150 mm | C18 | 3 µm | 120 Å | SH-200303-004X150 |
| Analytical | 4.6 × 150 mm | C18 | 5 µm | 120 Å | SH-200503-004X150 |
| Analytical | 4.6 × 250 mm | C18 | 3 µm | 120 Å | SH-200303-004X250 |
| Analytical | 4.6 × 250 mm | C18 | 5 µm | 120 Å | SH-200503-004X250 |
| Preparative | 10 × 150 mm | C18 | 5 µm | 120 Å | SH-200503-010X150 |
| Preparative | 10 × 250 mm | C18 | 5 µm | 120 Å | SH-200503-010X250 |
| Preparative | 21.2 × 150 mm | C18 | 5 µm | 120 Å | SH-200503-021X150 |
| Preparative | 21.2 × 250 mm | C18 | 5 µm | 120 Å | SH-200503-021X250 |
| Preparative | 30 × 150 mm | C18 | 5 µm | 120 Å | SH-200503-030X150 |
| Preparative | 30 × 250 mm | C18 | 5 µm | 120 Å | SH-200503-030X250 |
| Preparative | 50 × 150 mm | C18 | 5 µm | 120 Å | SH-200503-050X150 |
| Preparative | 50 × 250 mm | C18 | 5 µm | 120 Å | SH-200503-050X250 |
| Guard | 4.6 × 10 mm | C18 | 3 µm | 120 Å | SG-200303-004X10N (or D) |
| Guard | 4.6 × 10 mm | C18 | 5 µm | 120 Å | SG-200503-004X10N (or D) |
| Guard | 10 × 10 mm | C18 | 5 µm | 120 Å | SG-200503-010X10D |
| Guard | 21.2 × 10 mm | C18 | 5 µm | 120 Å | SG-200503-021X10N |