A Structured Portfolio
The SepaPrep HPLC portfolio includes a comprehensive range of reversed-phase, polar, and specialty stationary phases, aligned where applicable with established USP classifications. This structured offering simplifies column selection and facilitates method development, troubleshooting, and regulatory documentation.
Available chemistries include:
- • Universal reversed-phase columns for routine applications
- • Aqueous-compatible phases for polar analytes
- • High-density bonded phases for non-polar compounds
- • Low pH and high pH resistant columns for challenging conditions
- • HILIC and specialty phases for highly polar compounds
This portfolio-based approach enables efficient method optimization while maintaining consistent selectivity principles.
| Phase | USP Code | Particle Size (µm) | Pore Size (Å) | Specific Surface Area (m²/g) | Endcapping | Carbon Load (%) | pH Range |
| SepaPrep™ UNI Series - Universal Reversed-Phase Performance for Routine and Method-Development Workflows | |||||||
| C18 | L1 | 3 & 5 | 120 | 340 | Double | 14 | 1.5 - 9.0 |
| SepaPrep™ AQT Series - Reversed-Phase Columns Optimized for Highly Aqueous and Polar Separations | |||||||
| C18 | L1 | 3, 5 & 10 | 100 | 380 | Single | 18 | 1.5 - 9.0 |
| C8 | L7 | 5 | 100 | 380 | Single | 13 | 1.5 - 9.0 |
| SepaPrep™ LPS Series - Low-pH Stable Reversed-Phase Columns for Acidic and LC-MS Applications | |||||||
| C18 | L1 | 3, 5 & 10 | 150 | 200 | Non-endcapped | 12 | 0.8 - 7.5 |
| C18 (WP) | L1 | 5 | 300 | 80 | Non-endcapped | 5 | 0.8 - 7.5 |
| C8 | L7 | 5 | 150 | 200 | Non-endcapped | 7 | 1.0 - 7.5 |
| SepaPrep™ STR Series - Robust Reversed-Phase Columns Designed for Stable Performance and Contamination Resistance | |||||||
| C18 | L1 | 5 | 150 | 200 | Double | 15 | 1.5 - 9.0 |
| C8 | L7 | 5 | 150 | 200 | Single | 8 | 1.5 - 8.5 |
| SepaPrep™ CSH Series - Hybrid-Surface Reversed-Phase Columns for High-pH and Basic Compound Separations | |||||||
| C18 (H) | L1 | 5 & 10 | 100 | 380 | Double | 21 | 1.5 - 12.0 |
| C18 (L) | L1 | 3, 5 & 10 | 150 | 200 | Double | 14 | 1.5 - 12.0 |
| C8 (H) | L7 | 5 | 100 | 380 | Single | 14 | 1.5 - 12.0 |
| C8 (L) | L7 | 3 & 5 | 150 | 200 | Double | 10 | 1.5 - 12.0 |
| SepaPrep™ XDB Series - High-Density Bonded Phases for Strong Hydrophobic Retention and Robust Performance | |||||||
| C18 | L1 | 5 & 10 | 100 | 380 | Double | 18 | 1.5 - 9.0 |
| C18 (H) | L1 | 5 & 10 | 100 | 380 | Double | 22 | 1.5 - 10.0 |
| C8 | L7 | 5 | 100 | 380 | Single | 14 | 1.5 - 8.5 |
| Amino (NH₂) | L8 | 5 | 100 | 380 | Non-endcapped | 6 | 2.0 - 8.5 |
| Cyano (CN) | L10 | 5 | 100 | 380 | Single | 5 | 2.0 - 9.0 |
| Phenyl | L11 | 5 | 100 | 380 | Single | 12 | 1.5 - 9.0 |
| Silica | L3 | 5 | 100 | 380 | Non-endcapped | - | 1.0 - 7.0 |
| SepaPrep™ HILIC - Hydrophilic Interaction Chromatography for Highly Polar and Water-Soluble Compounds | |||||||
| HILIC | - | 5 & 10 | 100 | 380 | Non-endcapped | 8 | 2.0 - 8.0 |
Note: The maximum operating temperature is 60°C and the maximum pressure rating is 5,800 psi (40 MPa). For routine operation, pressure below 2,900 psi (20 MPa) is recommended.
Ordering Information
This section will guide you in building your SepaPrep™ HPLC column product number. Each number follows the structure outlined below.
[Column Type Code] - [Stationary Phase Code] - [Column Dimension Code]
Ex: SepaPrep™ UNI C18 HPLC Column, 5 µm, 120 Å, 4.6 x 150 mm: SH-200503-004X150
Ordering Information
This section will guide you in building your SepaPrep™ HPLC column product number. Each number follows the structure outlined below.
[Column Type Code] [Stationary Phase Code] [Column Dimension Code]
Ex: SepaPrep™ UNI C18 HPLC Column, 5 µm, 120 Å, 4.6 x 150 mm: SH-200503-004X150
[Column Type Code]
[Column Dimension Code]
* Add “N” at the end of the product number for non-direct connection / “D” for direct connection. | [Stationary Phase Code]
** Add “W” at the end of the product number. |
Available Column Formats
The table below outlines the available column formats by stationary phase series and particle size, covering analytical, preparative, and guard columns.
| ID x Length (mm) | UNI 3 µm | UNI 5 µm | AQT 3 µm | AQT 5 µm | AQT 10 µm | LPS 3 µm | LPS 5 µm | LPS 10 µm | STR 5 µm | CSH 5 µm | CSH 10 µm | XDB 5 µm | XDB 10 µm | HILIC 5 µm | HILIC 10 µm |
| Analytical Columns | |||||||||||||||
| 4.6 x 150 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| 4.6 x 250 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Preparative Columns | |||||||||||||||
| 10 x 150 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| 10 x 250 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| 21.2 x 150 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| 21.2 x 250 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Guard Columns | |||||||||||||||
| 4.6 x 10 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| 10 x 10 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| 21.2 x 10 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
* Preparative columns are available in C18 phase only. ** Preparative columns are available in C18, C8 and Silica phases only.
SepaPrep™ HPLC Accessories
The Importance of HPLC Guard Columns
Guard columns offer a simple, cost-effective way to protect analytical, semi-preparative, and preparative HPLC columns. By trapping particulates and strongly retained contaminants before they reach the main column, guard columns help preserve separation efficiency, maintain peak quality, and improve run-to-run reproducibility.
Over time, HPLC columns often show increased backpressure and reduced performance. In many cases, this is caused by contamination concentrated at the column inlet, while the remainder of the packed bed remains largely intact. Adding a guard column creates a sacrificial packed section upstream of the main column, helping prevent irreversible inlet damage, reduce downtime, and lower replacement costs.
Non-Direct-Connection Guard Column Holder
The non-direct-connection guard column holder is installed between the injector and the HPLC column using standard tubing and fittings. This traditional design offers broad compatibility with most commercial HPLC systems and column configurations.
| Guard Type | Description | Part Number |
| Analytical | Suitable for 4.6 x 10 mm guard column | NH-100 |
| Semi-Preparative | Suitable for 10 x 10 mm guard column | NH-150 |
| Preparative | Suitable for 21.2 x 10 mm guard column | NH-200 |
Direct-Connection Guard Column Holder
The direct-connection guard column holder is a reusable solution designed to connect directly to the inlet of the analytical column. Its hand-tight, tool-free installation eliminates the need for additional tubing and fittings, reduces dead volume, and helps maintain optimal chromatographic performance while simplifying installation and maintenance.
- • Direct-connection design with no additional connectors
- • Fast, tool-free, hand-tight installation
| Guard Type | Description | Part Number |
| Analytical | Suitable for 4.6 x 10 mm guard column | NH-100 |
| Semi-Preparative | Suitable for 10 x 10 mm guard column | NH-150 |
| Preparative | Suitable for 21.2 x 10 mm guard column | NH-200 |
Universal Direct-Connection Guard Column Kit
TEXT TO BE ADDED
| Guard Type | Description | Part Number |
| Analytical | Direct-connection Guard Column Holder, Model NH-100, 1 piece; C18 Guard Column, 2 pieces | DH-100KT |