SepaPrep™ LPS Series
Low-pH Stable Reversed-Phase Columns for Acidic and LC-MS Applications
Series Overview
The SepaPrep™ LPS Series is specifically engineered to deliver exceptional stability and reproducible performance under strongly acidic conditions, with LP referring to Low pH and S indicating enhanced stability, while remaining fully compatible with highly aqueous mobile phases. The stationary phase is intentionally non-endcapped and built on a sterically protected bonding architecture that shields critical siloxane linkages from acid-driven degradation.
This design enables reliable operation at pH values as low as 0.8, with very low phase bleed and excellent chromatographic stability. As a result, SepaPrep™ LPS columns are particularly well suited for LC-MS workflows, impurity profiling, and the separation of highly polar analytes under acidic conditions.
Key Features
Silica Characteristics
| Phase | USP Code | Particle Size | Pore Size | Specific Surface Area | Endcapping | Carbon Load | pH Range |
|---|---|---|---|---|---|---|---|
| C18 | L1 | 3, 5 & 10 µm | 150 Å | 200 m2/g | Non-endcapped | 12 % | 0.8 - 7.5 |
| C18 (WP) | L1 | 5 µm | 300 Å | 80 m2/g | Non-endcapped | 5 % | 0.8 - 7.5 |
| C8 | L7 | 5 µm | 150 Å | 200 m2/g | Non-endcapped | 7 % | 1.0 - 7.5 |
To maintain consistent performance and extend the working life of the column, it is recommended to operate the SepaPrep™ LPS within its optimal pH range of 1.0 to 4.0. The maximum operating temperature is 60°C and the maximum pressure rating is 5,800 psi (40 MPa). For routine operation, pressure below 2,900 psi (20 MPa) is recommended.
Ordering Information
| Column Type | ID × Length | Phase | Particle Size | Pore Diameter | Part Number |
|---|---|---|---|---|---|
| Analytical Columns | |||||
| Analytical | 4.6 × 150 mm | C18 | 3 µm | 150 Å | SH-220305-004X150 |
| Analytical | 4.6 × 150 mm | C18 | 5 µm | 150 Å | SH-220505-004X150 |
| Analytical | 4.6 × 150 mm | C18 (WP) | 5 µm | 300 Å | SH-220507-004X150 |
| Analytical | 4.6 × 150 mm | C8 | 5 µm | 150 Å | SH-110505-004X150 |
| Analytical | 4.6 × 250 mm | C18 | 3 µm | 150 Å | SH-220305-004X250 |
| Analytical | 4.6 × 250 mm | C18 | 5 µm | 150 Å | SH-220505-004X250 |
| Analytical | 4.6 × 250 mm | C18 (WP) | 5 µm | 300 Å | SH-220507-004X250 |
| Analytical | 4.6 × 250 mm | C8 | 5 µm | 150 Å | SH-110505-004X250 |
| Preparative Columns | |||||
| Preparative | 10 × 150 mm | C18 | 5 µm | 150 Å | SH-220505-010X150 |
| Preparative | 10 × 150 mm | C18 | 10 µm | 150 Å | SH-221005-010X150 |
| Preparative | 10 × 250 mm | C18 | 5 µm | 150 Å | SH-220505-010X250 |
| Preparative | 10 × 250 mm | C18 | 10 µm | 150 Å | SH-221005-010X250 |
| Preparative | 21.2 × 150 mm | C18 | 5 µm | 150 Å | SH-220505-021X150 |
| Preparative | 21.2 × 150 mm | C18 | 10 µm | 150 Å | SH-221005-021X150 |
| Preparative | 21.2 × 250 mm | C18 | 5 µm | 150 Å | SH-220505-021X250 |
| Preparative | 21.2 × 250 mm | C18 | 10 µm | 150 Å | SH-221005-021X250 |
| Preparative | 30 × 150 mm | C18 | 5 µm | 150 Å | SH-220505-030X150 |
| Preparative | 30 × 150 mm | C18 | 10 µm | 150 Å | SH-221005-030X150 |
| Preparative | 30 × 250 mm | C18 | 5 µm | 150 Å | SH-220505-030X250 |
| Preparative | 30 × 250 mm | C18 | 10 µm | 150 Å | SH-221005-030X250 |
| Preparative | 50 × 250 mm | C18 | 10 µm | 150 Å | SH-221005-050X250 |
| Guard Columns | |||||
| Guard | 4.6 × 10 mm | C18 | 3 µm | 150 Å | SG-220305-004X10N (or D) |
| Guard | 4.6 × 10 mm | C18 | 5 µm | 150 Å | SG-220505-004X10N (or D) |
| Guard | 10 × 10 mm | C18 | 5 µm | 150 Å | SG-220505-010X10D |
| Guard | 21.2 × 10 mm | C18 | 5 µm | 150 Å | SG-220505-021X10N |