SepaPrep™ HILIC
Hydrophilic Interaction Chromatography for Highly Polar and Water-Soluble Compounds
Series Overview
The SepaPrep™ HILIC is designed for separations where reversed-phase chromatography provides insufficient retention. Operating in hydrophilic interaction chromatography (HILIC) mode, analytes are separated based on increasing hydrophilicity using mobile phases composed of acetonitrile mixed with water and volatile buffer salts, with acetonitrile content typically ranging from 40 to 97%.
The SepaPrep™ HILIC stationary phase is based on a chemically bonded amide functionality, providing strong and reproducible hydrophilic interactions while maintaining excellent stability. This chemistry delivers selectivity that complements reversed-phase methods and supports the use of highly volatile, MS-friendly mobile phases, making the series particularly well suited for LC-MS applications.
Key Features
Silica Characteristics
| Phase | USP Code | Particle Size | Pore Size | Specific Surface Area | Endcapping | Carbon Load | pH Range |
|---|---|---|---|---|---|---|---|
| HILIC | - | 5 & 10 µm | 100 Å | 380 m2/g | Non-endcapped | 8 % | 2.0 - 8.0 |
To maintain consistent performance and extend the working life of the column, it is recommended to operate the SepaPrep™ HILIC within its optimal pH range of 2.0 to 8.0. The maximum operating temperature is 60°C and the maximum pressure rating is 5,800 psi (40 MPa). For routine operation, pressure below 2,900 psi (20 MPa) is recommended.
Ordering Information
| Column Type | ID × Length | Phase | Particle Size | Pore Diameter | Part Number |
|---|---|---|---|---|---|
| Analytical Columns | |||||
| Analytical | 4.6 × 150 mm | HILIC | 5 µm | 100 Å | SH-650502-004X150 |
| Analytical | 4.6 × 150 mm | HILIC | 10 µm | 100 Å | SH-651002-004X150 |
| Analytical | 4.6 × 250 mm | HILIC | 5 µm | 100 Å | SH-650502-004X250 |
| Analytical | 4.6 × 250 mm | HILIC | 10 µm | 100 Å | SH-651002-004X250 |
| Preparative Columns | |||||
| Preparative | 10 × 150 mm | HILIC | 5 µm | 100 Å | SH-650502-010X150 |
| Preparative | 10 × 150 mm | HILIC | 10 µm | 100 Å | SH-651002-010X150 |
| Preparative | 10 × 250 mm | HILIC | 5 µm | 100 Å | SH-650502-010X250 |
| Preparative | 10 × 250 mm | HILIC | 10 µm | 100 Å | SH-651002-010X250 |
| Preparative | 21.2 × 150 mm | HILIC | 5 µm | 100 Å | SH-650502-021X150 |
| Preparative | 21.2 × 150 mm | HILIC | 10 µm | 100 Å | SH-651002-021X150 |
| Preparative | 21.2 × 250 mm | HILIC | 5 µm | 100 Å | SH-650502-021X250 |
| Preparative | 21.2 × 250 mm | HILIC | 10 µm | 100 Å | SH-651002-021X250 |
| Preparative | 30 × 150 mm | HILIC | 5 µm | 100 Å | SH-650502-030X150 |
| Preparative | 30 × 250 mm | HILIC | 5 µm | 100 Å | SH-650502-030X250 |
| Preparative | 50 × 250 mm | HILIC | 10 µm | 100 Å | SH-651002-050X250 |
| Guard Columns | |||||
| Guard | 4.6 × 10 mm | HILIC | 5 µm | 100 Å | SG-650502-004X10N (or D) |
| Guard | 4.6 × 10 mm | HILIC | 10 µm | 100 Å | SG-651002-004X10N (or D) |
| Guard | 10 × 10 mm | HILIC | 5 µm | 100 Å | SG-650502-010X10D |
| Guard | 10 × 10 mm | HILIC | 10 µm | 100 Å | SG-651002-010X10D |
| Guard | 21.2 × 10 mm | HILIC | 5 µm | 100 Å | SG-650502-021X10N |
| Guard | 21.2 × 10 mm | HILIC | 10 µm | 100 Å | SG-651002-021X10N |