SepaPrep™ CSH Series
Hybrid-Surface Reversed-Phase Columns for High-pH and Basic Compound Separations
Series Overview
The SepaPrep™ CSH Series is built on advanced hybrid-silica surface technology, with CS referring to CoreShell and H indicating a hybrid architecture, combining a silica core with a protective hydrophobic layer. This architecture shields the silica matrix from alkaline degradation, enabling reliable operation under elevated pH conditions while maintaining high chromatographic efficiency.
By moderating excessive hydrophobic interactions and preserving fast mass transfer, the SepaPrep™ CSH Series delivers sharp peaks, high loading capacity, and excellent durability, making it particularly well suited for basic analytes, preparative separations, and demanding high-pH workflows.
Key Features
Silica Characteristics
| Phase | USP Code | Particle Size | Pore Size | Specific Surface Area | Endcapping | Carbon Load | pH Range |
|---|---|---|---|---|---|---|---|
| C18 (H) | L1 | 5 & 10 µm | 100 Å | 380 m²/g | Double | 21 % | 1.5 - 12.0 |
| C18 (L) | L1 | 3 & 5 µm | 150 Å | 200 m²/g | Double | 14 % | 1.5 - 12.0 |
| C8 (H) | L7 | 5 µm | 100 Å | 380 m²/g | Single | 14 % | 1.5 - 12.0 |
| C8 (L) | L7 | 3 & 5 µm | 150 Å | 200 m²/g | Double | 10 % | 1.5 - 12.0 |
To maintain consistent performance and extend the working life of the column, it is recommended to operate the SepaPrep™ CSH within its optimal pH range of 2.0 to 10.0. The maximum operating temperature is 60°C and the maximum pressure rating is 5,800 psi (40 MPa). For routine operation, pressure below 2,900 psi (20 MPa) is recommended.
Ordering Information
| Column Type | ID × Length | Phase | Particle Size | Pore Diameter | Part Number |
|---|---|---|---|---|---|
| Analytical Columns | |||||
| Analytical | 4.6 × 150 mm | C18 (H) | 5 µm | 100 Å | SH-230505-004X150 |
| Analytical | 4.6 × 150 mm | C18 (L) | 5 µm | 150 Å | SH-230507-004X150 |
| Analytical | 4.6 × 250 mm | C18 (H) | 5 µm | 100 Å | SH-230505-004X250 |
| Analytical | 4.6 × 250 mm | C18 (L) | 5 µm | 150 Å | SH-230507-004X250 |
| Preparative Columns | |||||
| Preparative | 10 × 150 mm | C18 (H) | 5 µm | 100 Å | SH-230505-010X150 |
| Preparative | 10 × 150 mm | C18 (H) | 10 µm | 100 Å | SH-231005-010X150 |
| Preparative | 10 × 250 mm | C18 (H) | 5 µm | 100 Å | SH-230505-010X250 |
| Preparative | 10 × 250 mm | C18 (H) | 10 µm | 100 Å | SH-231005-010X250 |
| Preparative | 21.2 × 150 mm | C18 (L) | 5 µm | 150 Å | SH-230507-021X150 |
| Preparative | 21.2 × 250 mm | C18 (L) | 5 µm | 150 Å | SH-230507-021X250 |
| Guard Columns | |||||
| Guard | 4.6 × 10 mm | C18 (H) | 5 µm | 100 Å | SG-230505-004X10N (or D) |
| Guard | 10 × 10 mm | C18 (L) | 5 µm | 150 Å | SG-230507-010X10 |
| Guard | 21.2 × 10 mm | C18 (L) | 5 µm | 150 Å | SG-230507-021X10 |