SepaPrep™ AQT Series
Reversed-Phase Columns Optimized for Highly Aqueous and Polar Separations
Series Overview
The SepaPrep™ AQT Series is engineered to deliver stable, reproducible reversed-phase performance under highly aqueous mobile phase conditions. The AQT designation refers to Aqueous Treatment and reflects the column’s enhanced stability in highly aqueous mobile phases, where conventional C18 phases often suffer from loss of retention or phase collapse. An advanced dual-layer surface treatment creates a hydrophilic interface on the silica substrate, preserving retention while maintaining excellent chromatographic efficiency.
By reducing residual surface acidity and improving silica purity, the SepaPrep™ AQT Series provides clean peak shapes and robust performance for polar and moderately polar analytes, even under 100 % aqueous conditions. This makes SepaPrep™ AQT columns a practical and reliable alternative to ion-pair chromatography in many applications.
Key Features
Maintains stable retention and efficiency under highly aqueous conditions without phase collapse, expanding method flexibility for polar compounds.
The engineered hydrophilic interface reduces secondary interactions and minimizes peak tailing, particularly for polar and basic analytes.
Enables efficient separation of polar compounds while simplifying method development and MS compatibility.
Silica Characteristics
| Phase | USP Code | Particle Size | Pore Size | Specific Surface Area | Endcapping | Carbon Load | pH Range |
|---|---|---|---|---|---|---|---|
| C18 | L1 | 3, 5 & 10 µm | 100 Å | 380 m2/g | Single | 18 % | 1.5 - 9.0 |
| C8 | L7 | 5 µm | 100 Å | 380 m2/g | Single | 13 % | 1.5 - 9.0 |
To maintain consistent performance and extend the working life of the column, it is recommended to operate the SepaPrep™ AQT within its optimal pH range of 2.0 to 6.0. The maximum operating temperature is 60°C and the maximum pressure rating is 5,800 psi (40 MPa). For routine operation, pressure below 2,900 psi (20 MPa) is recommended.
Ordering Information
| Column Type | ID × Length | Phase | Particle Size | Pore Diameter | Part Number |
|---|---|---|---|---|---|
| Analytical | 4.6 × 150 mm | C18 | 3 µm | 100 Å | SH-180302-004X150 |
| Analytical | 4.6 × 150 mm | C18 | 5 µm | 100 Å | SH-180502-004X150 |
| Analytical | 4.6 × 150 mm | C18 | 10 µm | 100 Å | SH-181002-004X150 |
| Analytical | 4.6 × 150 mm | C8 | 5 µm | 100 Å | SH-080502-004X150 |
| Analytical | 4.6 × 250 mm | C18 | 3 µm | 100 Å | SH-180302-004X250 |
| Analytical | 4.6 × 250 mm | C18 | 5 µm | 100 Å | SH-180502-004X250 |
| Analytical | 4.6 × 250 mm | C18 | 10 µm | 100 Å | SH-181002-004X250 |
| Analytical | 4.6 × 250 mm | C8 | 5 µm | 100 Å | SH-080502-004X250 |
| Preparative | 10 × 150 mm | C18 | 5 µm | 100 Å | SH-180502-010X150 |
| Preparative | 10 × 150 mm | C18 | 10 µm | 100 Å | SH-181002-010X150 |
| Preparative | 10 × 250 mm | C18 | 5 µm | 100 Å | SH-180502-010X250 |
| Preparative | 10 × 250 mm | C18 | 10 µm | 100 Å | SH-181002-010X250 |
| Preparative | 21.2 × 150 mm | C18 | 5 µm | 100 Å | SH-180502-021X150 |
| Preparative | 21.2 × 150 mm | C18 | 10 µm | 100 Å | SH-181002-021X150 |
| Preparative | 21.2 × 250 mm | C18 | 5 µm | 100 Å | SH-180502-021X250 |
| Preparative | 21.2 × 250 mm | C18 | 10 µm | 100 Å | SH-181002-021X250 |
| Preparative | 30 × 150 mm | C18 | 5 µm | 100 Å | SH-180502-030X150 |
| Preparative | 30 × 150 mm | C18 | 10 µm | 100 Å | SH-181002-030X150 |
| Preparative | 30 × 250 mm | C18 | 5 µm | 100 Å | SH-180502-030X250 |
| Preparative | 30 × 250 mm | C18 | 10 µm | 100 Å | SH-181002-030X250 |
| Guard | 4.6 × 10 mm | C18 | 3 µm | 100 Å | SG-180302-004X10N (or D) |
| Guard | 4.6 × 10 mm | C18 | 5 µm | 100 Å | SG-180502-004X10N (or D) |
| Guard | 10 × 10 mm | C18 | 5 µm | 100 Å | SG-180502-010X10D |
| Guard | 10 × 10 mm | C18 | 10 µm | 100 Å | SG-181002-010X10D |
| Guard | 21.2 × 10 mm | C18 | 5 µm | 100 Å | SG-180502-021X10N |