Column volume is approximately equal to dead volume (VM) when ignoring the additional volume in the tubings connecting the column with the injector and the detector.
Dead time (tM) is the time required for elution of an unretained component.
Dead volume (VM) is the volume of the mobile phase required for elution of an unretained component. Dead volume can be calculated by the following equation:VM =F0*tM.
Among the above equation, F0 is the flow rate of the mobile phase.
Post time: Jul-13-2022