For optimal purification with C18 flash columns, please follow these steps:
① Flush the column with 100% of the strong (organic) solvent for 10 – 20 CVs (column volume), typically methanol or acetonitrile.
② Flush the column with 50% strong + 50% aqueous (if additives are required, include them) for another 3 – 5 CVs.
③ Flush the column with the initial gradient conditions for 3 – 5 CVs.
Post time: Jul-13-2022