The flow cell of the detector module is contaminated by the sample which has strong UV absorption. Or it might be due to solvent UV absorption which is a normal phenomenon. Please do the following operation:
1. Remove the flash column and flush the system tubing with strongly polar solvent then followed by weakly polar solvent.
2. Solvent UV absorption problem: e.g. while n-hexane and dichloromethane (DCM) are employed as the eluting solvent, as the proportion of DCM increases, the baseline of chromatogram may continue to be below zero on Y-axis since the absorption of DCM at 254 nm is lower than that of n-hexane. In case of this phenomenon happens, we can handle it by clicking the “Zero” button on the separation running page in SepaBean App.
3.The flow cell of the detector module is heavily contaminated and needs to be cleaned ultrasonically.
Post time: Jul-13-2022