Amandla esixhobo kwaye ulinde ngokukhawuleza okukhawulezayo. Qiniseka ukuba inethiwekhi yenethiwekhi ye-IPAD ichanekile, kwaye i-router ixhotyiswa.
Jonga kwaye uqinisekise imeko ye-roer ukuqinisekisa ukuba i-iPad ingaqhagamshelwa kwi-router yangoku.
Ukulungelelaniswa kwenziwe xa ikholamu imanzi kwaye ijongeka iguqukile. Ngokwesiqhelo oku kunokwenziwa ngokutsiba 2 ~ 3 cvs zenqanaba leselula. Ngexesha lenkqubo yokulingana, ngamanye amaxesha sinokufumanisa ukuba ikholamu ayinakumanzi ngokupheleleyo. Le yinto eqhelekileyo kwaye ayiyi kulalanisa umsebenzi wokwahlukanisa.
Jonga ukuba i-tube Rack ibekwe ngokuchanekileyo kwindawo efanelekileyo. Xa oku kwenziwe, isikrini se-LCD kwi-tube rack kufuneka ibonise uphawu oluqhagamshelwe.
Ukuba i-tube rack iphosakele, umsebenzisi unokukhetha i-tube ye-tube yezezifiso ukusuka kuluhlu lwe-tube ye-tube kwi-printan app ukuze isetyenziswe okwethutyana. Okanye uqhagamshelane nenjineli yentengiso emva.
Jonga ukuba ibhotile yesilivere kukungabikho kwesolvent enxulumene nokugcwalisa isinyibilikisi.
Ukuba umgca weSolvent ugcwele isolvent, nceda ungakhathazeki. Ibubble yomoya ayichaphazeli ukwahlukana kwefleshi kuba akunakufikeleleka ngexesha lesampulu yokuqina. Ezi qabbs ziya kutsalwa ngokuthe ngcembe ngexesha lokwahlukana.
Nceda uvule isiciko sangasemva sesixhobo, ucoce i-piston piston rod nge-ethanol (uhlalutyo lwe-ethano okanye ngasentla), kwaye ujikeleze ipiston ngelixa i-piston iguquke kakuhle.
1. Isixhobo asiyi kuba nakho ukupeyimba izinyibilikisi xa iqondo lobushushu elapha ngaphezulu kwe-30 ℃, ngakumbi izinyibilikisi ezibilayo, ezinjengeDichloromethane okanye i-ether.
Nceda uqinisekise ukuba iqondo lobushushu lingaphantsi kwama-30 ℃.
I-2. Imozulu ithatha ipibhoyili ngelixa i-Instumumnet iphuma kusebenza ixesha elide.
Nceda udibanise i-ethanol rod ye-cemp yentloko ye-pump (uhlalutyo olucocekileyo okanye ngaphezulu) kwaye unyusa umgangatho wokuhamba ngaxeshanye. Isinxibelelanisi phambi kwempompo esonakalisiweyo okanye ikhululwe, oku kuya kubangela ukuba umgca uyokuvuza umoya.
3. Isinxibelelanisi phambi kwempompo eyonakalisiweyo okanye ikhululwe, iya kubangela ukuba umgca ucele umoya.
Nceda uqinisekise ukuba ngaba i-proxchactucy ikwimeko elungileyo.
I-valve yokuqokelela ivaliwe okanye iluphala. Nceda ubuyise ivalve ye-orlodid yeendlela ezintathu.
Ingcebiso: Nceda unxibelelane nenjineli yentengiso yasemva kokulungiswa ukuze ujongane nayo.
Coca intloko yentloko yeSolvent ngokupheleleyo ukuze ususe nayiphi na impatho, kungcono ukusebenzisa ukucoca i-ultrasonic.
1. Iseli yokuhamba komcuphi wangcoliswa.
2. Amandla asezantsi omthombo wokukhanya.
I-3. Impembelelo yempompo yempompo.
4. Ifuthe lobushushu be-cittocki.
5. Kukho amaqamza kwichibi lovavanyo.
Ikholamu okanye isiseko senqanaba leSigaba seSoftware.
Kwi-chromatography yokulungiselela, isixa esincinci sengxolo esisisiseko asinampembelelo encinci yokwahlukana.
1. Isinxibelelanisi se-tube ngasemva komatshini sikhululekile okanye sonakalisiwe; Buyisela isinxibelelanisi seTube;
I-2. Indlela yegesi yegesi yonakele. Faka endaweni yeValve yokujonga.
Emva kokwahluka, kuyimfuneko ukulinda imizuzu emi-3-5 ngaphambi kokuvala ukuqinisekisa ukuthembeka kweerekhodi zokuvavanya.
Ukulungelelaniswa kwekholamu kungayikhusela ikholamu ukuba ingonakaliswa sisiphumo sokukhwela xa isolvent sifula ngokukhawuleza kwikholamu. Ngelixa i-silica iyoyileyo yangaphambi kokuba ifakwe kwikholamu inxibelelana neSolvent okokuqala ngexesha lokwahlukana, ubushushu obuninzi bunokukhululwa ngakumbi xa i-solvent iqhuma kwinqanaba lokuhamba okuphezulu. Olu bushushu lunokubangela umzimba wekholamu ukuba usebenze kwaye ke ngoko ke ugcino oluvela kwikholamu. Ngamanye amaxesha, obu bushushu bunokuyonakalisa isampulu enobushushu.
Inokubangelwa kukungabikho kweoyile ethambisa ioyile kwishayile ejikelezayo yempompo.
Umthamo opheleleyo we-tubing yenkqubo, isinxibelelanisi kunye negumbi lokudibanisa limalunga ne-25 ml.
Iseli yokuhamba kwemodyuli yokufuma ingcoliseka yisampulu enolwazi oluqinileyo lwe-UV. Okanye kunokubangelwa kukucombulula i-uv Nceda wenze le ngqesho ilandelayo:
1. Susa ikholamu yeFlash kwaye ugungxule inkqubo ityhubhu ene-polar ye-polar ilandelwa yi-Solver ebuthathaka.
I-2. Songa iNgxaki ye-Ackerve: Umzekelo ngelixa i-N-Hexane kunye neDichloromethane (i-DCM) iqeshwa i-SCRM kwi-DCM kwi-254 NM iphantsi kune-N-Hexane. Kwimeko yento eyenzekayo, singakwazi ukuyiphatha ngokucofa iqhosha "zero" kwi-Supriction Page ewahlelayo.
3. Iseli yokuhamba kwemodyuli yokufumanisa ingcolisekile kwaye kufuneka icocwe icocekile.
Inokubangelwa kukuba uqhagamshelo kwintloko yekholamu kwaye kwinxalenye yesiseko ityhutywe ngokwenza isinyibilikisi ukuze izivakalisi zibambekile.
Umsebenzisi unokunyusa ngesandla intloko yekholamu ngokusebenzisa amandla amancinci. Xa intloko yabangabambanga iKholamu iphakanyiselwe ekuphakameni okuthile, intloko yabangamkizi kufuneka ikwazi ukushukunyiswa ngokuchukumisa amaqhosha kuyo. Ukuba intloko yekholamu ayinakuphakanyiswa ngesandla, umsebenzisi kufuneka anxibelelane nenkxaso yezobugcisa yendawo.
Enye indlela yongxamiseko: Umsebenzisi unokufaka ikholamu ngaphezulu kwentloko yekholamu endaweni yoko. Isampulu yereyitha inokufakwa ngqo kwikholamu. Isampulu yesampulu eqinileyo inokufakwa ngaphezulu kwekholam yokwahlulahlula.
1. Amandla asezantsi omthombo wokukhanya;
2. Iphuli yedini ingcolile; I-ITUATRE, akukho cala libonakalayo okanye i-Peak ebonakalayo incinci ngokwahlula-hlulwa, amandla abonakalayo abonisa ixabiso elingaphantsi kwe-25%.
Nceda ugungxule ityhubhu enesimo esifanelekileyo kwi-10ml / min ye-30min kwaye ugcine i-spectrium.if akukho tshintsho kumthombo wokukhanya, nceda ubeke endaweni ye-Deuterium Lamp; Ukuba i-spectrum itshintshiwe, ichibi lesasazwe lingcolile, nceda uqhubeke nokucoca ngesifo esifanelekileyo.
Nceda ujonge ityhubhu kunye nomnxibelelanisi rhoqo.
Ukufunyanwa kwabekwe kwi-wavlengd kwi-wavnength engaphantsi kwe-245 nts ukuba i-ithyl acetate inolwazi olubonakalayo kwi-245nm. Ukuhamba kwesiseko kuya kuba yinto enkulu xa i-ethyl acetate isetyenziswa njengeSolvent ye-Elvent kwaye sikhetha i-220 NM njengoko ifunyanwe i-waveength yavelen.
Nceda utshintshe ukufumanisa i-wavelength. Kucetyiswa ukukhetha i-254nm njengoko ifumene i-wavevength. Ukuba i-220 NM kuphela kwe-wavelength kuphela yokufunyanwa kwesampulu, umsebenzisi kufuneka aqokelele i-auent onesifo sokugweba kunye ne-Solvent egqithisileyo inokuqokelelwa kule meko.
Coca intloko yentloko ye-Solvent ngokupheleleyo ukususa nayiphi na impatho. Sebenzisa i-ethanol okanye i-isopropanol yokuqhubela phambili le nkqubo ngokupheleleyo ukuphepha iingxaki zokuzila izilwanyana.
Ukucoca intloko ye-Solvent, ukonakalisa icebo lokucoca kwintloko yecebo lokucoca kwaye ucoce ngebrashi encinci. Emva koko hlamba icebo lokucoca ne-ethanol kwaye lome ngayo. Faka kwakhona intloko yecebo lokusebenzisa elizayo.
Nokuba ukutshintshela ukusuka ekwinqanaba lesigaba esiqhelekileyo lokwahlulahlula-hlulwa kwakhona okanye kwi-Ethanol okanye i-Isopropanol kufuneka isetyenziswe njengesinyibilikisi sokutshisa ngokupheleleyo.
Kucetyiswa ukuseta ireyithi yokuhamba nge-40 ml / min ukuze igungxule imigca yeSolvent kunye nazo zonke iityhubhu zangaphakathi.
Nceda uphinde uphinde uphinde ufake amazantsi omnini wekholamu emva kokukhulula isikere.
I-1. Inkqubo yokuhamba kwenkqubo iphezulu kakhulu kwikholam yeflash yangoku.
2. Isampulu inomhlaba ombi kunye nokungabinamthi kwiSigaba seSoftware, ngaloo ndlela sikhokelela kwi-bloung block.
I-3. Esinye isizathu sibangela ukuba i-bloung block.
Imo engqongileyo imanzi kakhulu, okanye i-solvent ukuvuza ngaphakathi komnini wekholamu kubangela isekethe emfutshane. Nceda ubushushu bomgcini wekholamu ngokufanelekileyo ngumsi womisa iinwele okanye umpu oshushu emva kwento.
I-Solvent Ukuvuza ukuvuza kunokubangelwa kwinqanaba lokulungisa kwibhotile yenkunkuma kungaphezulu kunendawo yokuphakama kwisiseko sekholamu.
Beka ibhotile yenkunkuma engezantsi kweqonga lezixhobo, okanye ngokukhawuleza uhambe phantsi umnini wekholamu emva kokususa ikholamu.
Lo msebenzi wokucoca yenzelwe ukucoca umbhobho wenkqubo ngaphambi kokwahlukana. Ukuba "ukucoca emva kokuba kwenziwe emva kokwahlukana kokugqibela, eli nyathelo lingatkitywa. Ukuba ayenziwanga, kuyacetyiswa ukwenza le nyathelo lokucoca njengoko kuyalelwe yinkqubo eshukumayo.