Ikholam yeSepaFlash™

Santai Solvent Lines Kit & Accessories

Hayi, i-silica ene-end-capped ayinyibiliki kuyo nayiphi na i-solvent esetyenziswa ngokuqhelekileyo.

Ukucocwa ngeyona nto iphezulu ngeC18 flash columns, nceda ulandele la manyathelo:
① Gubha ikholamu nge-100% ye-solvent eyomeleleyo (organic) ye-10 - 20 CVs (umthamo wekholomu), ngokuqhelekileyo i-methanol okanye i-acetonitrile.
② Gubha ikholamu nge-50% eqinile + i-50% yamanzi (ukuba izongezo ziyafuneka, zibandakanye) kwenye i-3 - 5 ye-CV.
③ Gubha ikholamu ngeemeko zokuqala zodidi lwe-3 – 5 CVs.

Ubukhulu bekholomu phakathi kwe-4g kunye ne-330g, isinxibelelanisi esiqhelekileyo seLuer sisetyenziswa kule miqolo ye-flash.Kubukhulu bekholamu ye-800g, 1600g kunye ne-3000g, iiadaptha zokudibanisa ezongezelelweyo kufuneka zisetyenziselwe ukunyuswa kwezi kholamu ezinkulu zombane kwi-flash chromatography system.Nceda ujonge uxwebhu Santai Adapter Kit for 800g, 1600g, 3kg Flash Columns ukuze ufumane iinkcukacha ezithe vetshe.

Kwikholamu yesigaba esiqhelekileyo, kucetyiswa ukuba kusetyenziswe isigaba seselula apho umlinganiselo we-methanol ungadluli kwi-25%.

Ngokuqhelekileyo, kucetyiswa ukuba kusetyenziswe isigaba esihambayo apho umlinganiselo we-solvents polar ungadluli kwi-5%.Izinyibilikisi zepolar ziquka iDMSO, iDMF, THF, TEA njl.

Ukulayishwa kwesampulu eqinileyo yindlela eluncedo yokulayisha isampuli ukuba ihlanjululwe kwikholamu, ngakumbi kwiisampuli zokunyibilika okuphantsi.Kule meko, i-ILOK flash cartridge lukhetho olufanelekileyo kakhulu.
Ngokuqhelekileyo, isampuli inyibilika kwi-solvent efanelekileyo kwaye ibhengezwe kwi-adsorbant eqinileyo enokuthi ifane nesetyenziswe kwiikholamu ezikhanyayo, kubandakanywa umhlaba we-diatomaceous okanye i-silica okanye ezinye izinto.Emva kokususwa / ukuphuphuma kwe-solvent eshiyekileyo, i-adsorbent ibekwa phezu kwekholamu ezaliswe ngokuyinxenye okanye kwikhatriji engenanto yokulayisha eqinileyo.Ngolwazi oluthe vetshe, nceda ujonge kuxwebhu iLOK-SL Cartridge User Guide ngeenkcukacha ezithe vetshe.

Umthamo wekholomu uphantse ulingane nomthamo ofileyo (VM) xa ungayihoyi ivolumu eyongezelelweyo kwiityhubhu ezidibanisa ikholamu kunye ne-injector kunye ne-detector.

Ixesha elifileyo (tM) lixesha elifunekayo kuhlaziyo lwecandelo elingagcinwanga.

Umthamo ofileyo (i-VM) ngumthamo wesigaba esiphathwayo esifunekayo ukuze kuveliswe icandelo elingagcinwanga.Umthamo ofileyo ungabalwa ngale equation ilandelayo: VM =F0*tM.

Phakathi kwe-equation engentla, i-F0 yizinga lokuhamba kwesigaba esihambayo.

Hayi, i-silica ene-end-capped ayinyibiliki kuyo nayiphi na i-solvent esetyenziswa ngokuqhelekileyo.

Iikholamu ze-silica flash ziyalahlwa kwaye zisetyenziselwa enye, kodwa ngokuphathwa ngokufanelekileyo, iikhatriji ze-silica zingaphinda zisetyenziswe ngaphandle kokuncama ukusebenza.
Ukuze isetyenziswe kwakhona, ikholomu ye-silica flash kufuneka yomiswe ngokulula ngomoya ocinezelweyo okanye igutyungelwe kwaye igcinwe kwi-isopropanol.

Ugcino olululo luya kuvumela ukuba kuphinde kusetyenziswe iikholamu zeFlash zeC18:
• Ungaze uvumele ikholamu yome emva kokuyisebenzisa.
• Susa zonke izilungisi zendalo ngokugungxula ikholamu nge-80% yemethanol okanye i-acetonitrile emanzini kangange-3 – 5 CVs.
• Gcina ikholamu kwesi sinyibilikisi sokugungxulwa sikhankanywe ngasentla nesifakela isiphelo endaweni.

Kwiikholomu ezinkulu zesayizi ngaphezu kwe-220g, umphumo we-thermal uyabonakala kwinkqubo yokulingana kwangaphambili.Kunconywa ukuseta izinga lokuhamba kwi-50-60% yezinga lokuhamba eliphakanyisiweyo kwinkqubo yokulinganisa kwangaphambili ukuphepha umphumo ocacileyo we-thermal.

Impembelelo ye-thermal ye-solvent edibeneyo ibonakala ngakumbi kune-solvent enye.Thatha i-solvent system cyclohexane/ethyl acetate njengomzekelo, kucetyiswa ukuba usebenzise i-100% ye-cyclohexane kwinkqubo yokulingana kwangaphambili.Xa ulungelelwaniso lwangaphambili lugqityiwe, uvavanyo lokwahlula lunokwenziwa ngokwenkqubo yokunyibilikisa esetwe kwangaphambili.