Amandla ethuluzi bese ulinda i-silica yayo esheshayo "Readbare Silica izoqala ukuncibilikisa ngesisombululo se-ph 9, yize i-solvent iyedlula i-PH. ekugcineni-i-capped.y ". Qiniseka ukuthi ukuxhumana kwenethiwekhi ye-iPad kulungile, futhi i-router inikwa amandla.
Ngokuhlanjululwa okuhle nge-C18 Flash Columns, sicela ulandele lezi zinyathelo:
① Flush ikholomu nge-100% ye-solvent eqinile (ye-organic) ye-solvent yama-10 - 20 ama-CVs (ivolumu yekholomu), imvamisa ye-methanol noma i-acetonitrile.
② Flush ikholomu nge-50% enamandla + 50% aqued (uma izengezo zidingeka, zibandakanya nazo) kwamanye ama-3 - 5 ama-CV.
③ Flush ikholomu ngezimo zokuqala ze-gradient ama-3 - 5 ama-CV.
Ngosayizi wekholomu phakathi kwe-4G ne-330G, isixhumi esijwayelekile se-LEERS sisetshenziswa kulezi zinkundla ze-flash. Ngosayizi wekholomu ka-800g, 1600g ne-3000g, ama-adap we-Connector angeziwe kufanele asetshenziselwe ukukhweza lezi zinhle ezinkulu ze-flash kuhlelo lwe-chromatography. Uyacelwa ukuthi ubheke kudokhumenti ye-Santai Adapter Kit ye-800g, 1600g, amakholomu we-3kg flash ukuthola eminye imininingwane.
Kwikholamu yesigaba ejwayelekile, kunconywa ukusebenzisa isigaba se-mobile lapho isilinganiso se-methanol singadluli ku-25%.
Ngokuvamile, kunconywa ukusebenzisa isigaba se-mobile lapho isilinganiso se-polar solvents esingadluli ku-5%. I-polar solvents ifaka i-DMSO, DMF, THF, itiye njll.
Ukulayisha isampula eqinile kuyindlela ewusizo ukulayisha isampula ukuze ihlanzwe kwikholamu, ikakhulukazi kumasampula we-solubity ephansi. Kulokhu, iLok Flash Carthridge iyisinqumo esifanele kakhulu.
Ngokuvamile, isampula lichithwa kwi-solvent efanelekile futhi i-adsorbed ku-adsorbant eqinile engafana ne-flash colomns, kufaka phakathi umhlaba we-diatomaceous noma ezinye izinto. Ngemuva kokususwa / ukuhwamuka kwe-solvent eyakhayo, ama-adsorbent afakwa ngaphezulu kwekholomu egcwele ngokwengxenye noma ebhokisini eliqinile eliqinile. Ngemininingwane enemininingwane eminingi, sicela ubheke kudokhumenti i-ILok-SL Cartridge Widle Sindies ukuthola eminye imininingwane.
IVolumu yekholomu icishe ilingane nevolumu efile (VM) lapho inganaki ivolumu eyengeziwe kumagama axhumanisa ama-tubings axhuma ikholomu nesijobelelo kanye nomtshina.
Isikhathi Esifile (i-TM) isikhathi esidingekayo sokuqedwa kwengxenye engafundisiwe.
IVolumu Efile (VM) ivolumu yesigaba seselula esidingekayo ekuqedeni ingxenye engafakwanga. Ivolumu efile ingabalwa yi-equation elandelayo: VM = F0 * TM.
Phakathi kokulingana okungenhla, i-F0 yisilinganiso sokugeleza kwesigaba seselula.
Cha, i-silica ephelile eboshwe i-infoluble kunoma iyiphi i-organic solvent evame ukusetshenziswa.
Amakholomu we-Silica Flash asetshenziswa futhi asetshenziswa okukodwa, kepha ngokuphathwa okufanele, ama-cartridge ama-silica angasetshenziswa ngaphandle kokuzidela ukusebenza.
Isitoreji esifanele sizovumela amakholomu we-C18 Flash ukuthi asetshenziswe kabusha:
• Ungalokothi uvumele ikholomu ukuthi yome ngemuva kokusebenzisa.
• Susa wonke ama-organic modifiers ngokufaka ikholomu nge-80% ye-methanol noma i-acetonitritle emanzini ama-3 - 5 ama-CV.
• Gcina ikholomu kuleli ngenhla elishiwo ngenhla elibunjiwe ngokufakelwa kokuphela endaweni.
Kumakholomu amakhulu asayizi ngaphezulu kwama-220g, umphumela we-thermal usobala kwinqubo yokulingana kwangaphambili. Kunconywa ukusetha isilinganiso sokugeleza ku-50-60% wesilinganiso sokugeleza esiphakanyisiwe kwinqubo yangaphambi kokulingana ukugwema umphumela osobala we-thermal.
Umphumela we-thermal we-solvent exubile usobala ukwedlula i-solvent eyodwa. Thatha uhlelo lwe-solvent cyclohexenane / ethyl acetate njengesibonelo, kuphakanyiswa ukuthi usebenzise i-100% cyclohekhali yenqubo yangaphambi kokulingana. Lapho kuqedwa ukulingana kwangaphambilini, ukuhlolwa kwehlukana kungenziwa ngokuya ngohlelo lwe-preset solvent.
NgeSepaflashTMAmakholomu aphezulu we-STADAST Series, izixhumi ezisetshenzisiwe zikhiya i-LEER-LICK InLe and Luer-Sking. Lawa makholomu angafakwa ngqo ezinhlelweni ze-dinkiflash zika-Ishini.
Ngochungechunge lweSepaflash HP, uchungechunge oluhlanganisiwe noma amakholomu we-Iloktm Series, izixhumi ezisetshenzisiwe zikhiya i-LEER-LOCK in and Luer-Khiya ngaphandle. Lawa makholomu nawo angafakwa ezinhlelweni ze-Dinkiflash ze-Ishini nge-Adaptha eyengeziwe. Ngemininingwane yalawa ma-adaptha, sicela ubheke kudokhumenti Santai Adapter Kit nge-800g, 1600g, 3kg Flash Columns.
I-Parameper Colommum Volul (CV) iwusizo ikakhulukazi ukunquma izinto ezikade. Amanye amakhemikhali acabanga ivolumu yangaphakathi ye-cartridge (noma ikholomu) ngaphandle kokupakisha izinto ngaphakathi yivolumu yekholomu. Kodwa-ke, umthamo wekholomu engenalutho akuyona i-CV. I-CV yanoma iyiphi ikholamu noma i-cartridge yivolumu yesikhala esingahlali ngokusetshenziswa kwangaphambi kwekholomu. Le ncwadi ifaka phakathi ivolumu ye-interstitial (ivolumu yesikhala esingaphandle kwezinhlayiya ezigcwele) kanye ne-poresity yangaphakathi yezinhlayiyana (ivolumu ye-pore).
Amakholomu we-alumina flash angeyona enye indlela lapho amasampula ebucayi futhi ethambekele ekunciphiseni e-silica gel.
Ingcindezi engemuva yekholomu ye-flash ihlobene nosayizi wezinhlayiyana zezinto ezigcwele. Izinto ezigcwele ezigcwele usayizi omncane wezinhlayiyana kuzoholela ekucindezelweni okuphezulu emuva kwekholomu ye-flash. Ngakho-ke izinga lokugeleza kwesigaba se-mobile esisetshenziswa ku-flash chromatography kufanele sehliswe ngokufanele ukuze kuvikeleke uhlelo lwe-flash ukuthi liyeke ukusebenza.
Ingcindezi yangemuva yekholomu ye-flash nayo iyalingana nobude bekholomu. Umzimba wekholomu ende uzoholela ekucindezelweni okuphezulu kwekholomu ye-flash. Ngaphezu kwalokho, ingcindezi engemuva yekholomu ye-flash ilingana kakhulu ne-ID (ububanzi obungaphakathi) bomzimba wekholomu. Ekugcineni, ingcindezi engemuva yekholomu ye-flash ilingana nokubonakala kwesigaba seselula esisetshenziswa ku-flash chromatography.