No ka hoʻomaʻemaʻe maikaʻiʻana me nā kolamu C18, eʻoluʻolu e hahai i kēia mau hana:
① flash i ka kolamu me ka 100% o ka ikaika (a me keʻano) no 10 - 20 cvs (20 coll (column volume)
② Hoʻopili i ka kolamu me 50% ikaika + 50% Aquouous (inā e koiʻia, e hoʻopili iā lākou) no kekahi 3 - 5 CVS.
③ E lele i ka kolamu me nā kūlana gradient mua no 3 - 5 cvs.
Post Time: Jul-13-2022